Fig. 5

Restoration of _mCa²⁺ efflux improves mitochondrial function in AD mutant mice. a Representative traces for _mCa²⁺ retention capacity (CRC). b Percent change in CRC of 3xTg-AD × Camk2a-Cre and 3xTg-AD × NCLX^fl/fl × Camk2a-Cre vs. Camk2a-Cre control. c Representative trace for CRC in Camk2a-tTA, 3xTg-AD × Camk2a-tTA and 3xTg-AD × TRE- NCLX × Camk2a-tTA mice. d Percent change in _mCa²⁺ retention capacity of 3xTg-AD × Camk2a-tTA and 3xTg-AD × TRE- NCLX × Camk2a-tTA vs. Camk2a-tTA control. e DHE staining for ex vivo detection of superoxide production in freshly prepared cortical and hippocampal sections from 12 months old mice. f, g DHE fluorescent intensity, percent change vs. 3xTg-AD × Camk2a-Cre controls. h DHE staining for ex vivo detection of superoxide production in freshly prepared cortical and hippocampal sections from 12 months old mice. i, j Quantification of DHE fluorescent intensity, percent change vs. 3xTg-AD × Camk2a-tTA controls. k Representative images of hippocampal 4-HNE immunohistochemistry to detect lipid peroxidation in 12 months old mice. l Percent change in 4-HNE-integrated optical density area corrected to 3xTg-AD × Camk2a-Cre controls. m Representative images of hippocampal 4-HNE immunohistochemistry to detect lipid peroxidation in 12 months old mice. n Percent change in 4-HNE-integrated optical density area corrected with 3xTg-AD × Camk2a-tTA controls. o Mitochondrial DNA (mtDNA)/nuclear DNA (nDNA) ratio in tissue isolated from the cortex of 2 and 12 months old mice, fold change vs. 2 months old Camk2a-Cre controls. p mtDNA/nDNA ratio in tissue isolated from the brain cortex of 2 and 12 months old mice expressed as fold change vs. 2 months old Camk2a-tTA controls. (n = individual dots shown for each group in all graphs. All data presented as mean ± SEM; ****p < 0.001, **p < 0.01, *p < 0.05; one-way ANOVA with Sidak's multiple comparisons test.) Source data are available as a Source Data file