Fig. 8 | Nature Communications

Fig. 8

From: Modulation of actin polymerization affects nucleocytoplasmic transport in multiple forms of amyotrophic lateral sclerosis

Fig. 8The alternative text for this image may have been generated using AI.

Formin mDia1 overexpression rescues nuclear import defects. a, b Time-lapse images and quantification of S-mCherry nuclear import dynamics upon treatment with LMB in cortical neurons expressing GFP or GFP-mDia1 (green) and V5-tagged PFN1 (white). S-mCherry levels are shown as 16-bit heat map. Dashed lines indicate the nucleus. Cells were post-fixed and stained to verify the expression of V5-PFN1 constructs (white). c, d Regression analysis of S-mCherry nuclear import kinetics (c) and percentage of non-responder cells (d). DAPI (blue) was used to detect the nucleus and assess cell health. Scale bars: 10 µm. Bars are mean ± SEM. ***p < 0.001. N = 43–50 cells from 4 independent experiments. See also Supplementary Table 1 for details on statistics

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