Fig. 5
Ripk1K376R/− mice are viable but display systemic inflammation. a Representative whole-mount dark field images of E13.5 embryos with indicated genotypes. b Ripk1+/−, Ripk1K376R/K376R, and Ripk1K376R/− MEFs were treated with T, TS, TSZ, TC, and TCZ, respectively. Cell viability was determined using the CellTiter-Glo kit. Data are represented as mean ± SEM of MEFs derived from three embryos. P values were determined by Student’s t-test (*p < 0.05, **p < 0.01, ***p < 0.001). Abbreviations are as follows: UT untreated, TSZ TNF-α (20 ng/ml)+Smac mimetic(100nM) +zVAD(20 μM), TSZN TNF-α+Smac mimetic +zVAD+Necrostatin-1(20 μM), TC TNF-α+CHX(20 ng/ml), TCZ TNF-α+CHX +zVAD. Source data are provided as a Source Data file. c Ripk1+/−, Ripk1K376R/K376R, and Ripk1K376R/− MEFs were treated with TS(6h), TSZ(2h), TC(3h), TCZ(1h) with or without Nec-1s. Western blottings of indicated proteins were shown. d Representative 8-week-old Ripk1K376R/K376R mouse and littermate control. e Representative hematoxylin and eosin (H&E)-stained tissues from 4-week-old animals of the indicated genotypes. (Scale bars, 100 μm). f Plot of weight of littermate Ripk1+/− and Ripk1K376R/− mice. g Different cell subsets in spleens and LNs from 6-week-old mice with indicated genotypes were determined by flow cytometry using following markers: B cells (CD19+ or B220+), T cells (CD3+), Monocytes and macrophages (CD11b+), Granulocytes (Gr-1+). Data are represented as mean ± SEM (n = 3/genotype). P values were calculated by Student’s t-test (*p < 0.05, **p < 0.01, ***p < 0.001). Source data are provided as a Source Data file
