Fig. 6

Tnfr1^−/−Ripk1^K376R/K376R mice die postnatally due to RIPK3-dependent inflammation. a Whole-mount dark field images of embryos with indicated genotypes. Images are representative of embryos from E13.5 (n = 8) and E14.5 (n = 9). b Representative 1-week-old Tnfr1^−/−Ripk1^K376R/K376R mice and littermate control. c Expected and observed frequency of genotypes in offspring at weaning from crosses of Tnfr1^−/−Ripk1^K376R/+. d Quantitative RT-PCR analysis of the mRNA expression of cytokines in lungs and intestines from 1-week-old pups with indicated genotypes. Data are represented as mean ± SEM (n = 3). Source data are provided as a Source Data file. e Representative H&E stained tissues from 1-week-old Tnfr1^−/−Ripk1^K376R/K376R and littermate control. Scale bars, 100 μm. f Representative 8-week-old Tnfr1^−/−Ripk3^−/−Ripk1^K376R/K376R and its littermate controls. g Representative H&E stained tissues from 8-week-old Tnfr1^−/−Ripk3^−/−Ripk1^K376R/K376R and littermate control. Scale bars, 100 μm. h Kaplan-Meier plot of mouse survival from intercrosses of Tnfr1^−/−Ripk1^K376R/+Ripk3^+/−