Fig. 2
From: Zika virus replicates in adult human brain tissue and impairs synapses and memory in mice
ZIKV replicates and targets memory-related areas in adult mouse brain. a Adult mice received an infusion of 105 PFU ZIKV, UV-inactivated ZIKV or mock medium into the cerebral lateral ventricle. b At the indicated days post-infection (dpi), brains were processed for determination of ZIKV RNA levels by qPCR (F(6,49) = 25.22; *p < 0.0001 comparing 6 dpi to other time points, one-way ANOVA followed by Bonferroni; N = 10 (6 dpi); 9 (1 and 60 dpi); 8 (4 and 30dpi); 7 (15dpi) and 5 (45dpi). c Body weight measured up to 35 dpi (F(2,27) = 18.11; *p < 0.0001 compared to mock-infused mice, repeated measures ANOVA followed by Bonferroni; N = 10 mice/group; graph represents one of two independent experiments). d, e Distinct CNS (d) or peripheral (e) structures were isolated at 6dpi and processed for determination of ZIKV RNA levels by qPCR. (In d F(4,38) = 3.096; * indicates: p = 0.0205 Hpc vs FC, p = 0.0125 Hpc vs Hpt, p = 0.0137 Hpc vs CB, p = 0.0105 Hpc vs SC, one-way ANOVA followed by Bonferroni. In e: F(4,36) = 4.014; *p = 0.0206 SN vs DRG, p = 0.0181 SN vs SPL, p = 0.0112 SN vs LVR, p = 0.0115 SN vs Testes, one-way ANOVA followed by Bonferroni; N = 6 (Hpc hippocampus); 9 (SN sciatic nerve); 8 (Str striatum, Hpt hypothalamus, SC spinal cord, DRG dorsal root ganglion, SPL spleen, LVR liver and Testes); 7 (FC and CB). Bars represent means ± SEM. Symbols represent individual mice. f At 6 dpi, brains were processed for immunohistochemistry. (g–o) Representative ZIKV immunolabeling (NS2B protein) in distinct brain regions from ZIKV-infected mice: hippocampus (g), CA1 hippocampal subregion (h, i), dentate gyrus (DG) (j), striatum (k, l), frontal cortex (m), thalamus (Th) (n), sub-ventricular zone (SVZ) (o). LV lateral ventricle. Arrows point to areas of necrosis. p–x Representative double immunofluorescence images for ZIKV (NS2B protein, red), NeuN (p–r), GFAP (s–u), or F4/80 (v–x) (green) in the CA1 hippocampal region of ZIKV-infected mice (N = 4). Scale bars = 200 μm (g, k), 40 μm (h–j), 50 μm (l–o), 10 μm (p–r), 15 μm (s–x). Source data from panels b–e are provided as Source Data File
