Fig. 4
From: In vitro fusion of single synaptic and dense core vesicles reproduces key physiological properties
Munc13 dependence of secretory vesicle kinetics. Cumulative distribution functions for synaptic vesicles (a), dense-core vesicles (b), insulin vesicles (c), dense-core vesicles lacking endogenous CAPS-1/2 (previously described in ref. 3) (d). All kinetics are triggered from a docked state in the presence of syntaxin-1a:dSNAP-25, Munc18, complexin-1 without (black), or with (purple) 0.2 μM of the C1C2MUN domain fragment of Munc13. Summary statistics for each condition are shown in Supplementary Table 2. e Bar graph for the total amount of fusion in the presence of 100 µM EDTA, 100 µM calcium, or 100 µM calcium in the presence of 0.2 µM C1C2MUN domain of Munc13 for each condition. Error bars represent standard errors of the mean
