Fig. 2

High-dimensional analysis of natural killer (NK) cells responding (Ki67⁺ and Ki67⁻) during acute dengue virus (DENV) infection. a Barnes–Hut stochastic neighbor embedding (Bh-SNE) maps on total CD56^bright NK cells and separated for responding (Ki67⁺) and non-responding (Ki67⁻) cells. Residual plot showing the difference between responding and non-responding maps. Analysis is based on data from three representative patients at the acute phase of infection with 5000 events per subset and patient. b Relative expression intensities of the 11 indicated parameters that were used in the SNE analysis. Phenotypes within red circles are more common in responding NK cells and vice versa for blue circles in non-responding NK cells. c, d Similar analysis as in a, b but for CD56^dim NK cells. e Representative flow cytometry staining for Bcl-2 and Ki67 expression of T cells, CD56^bright NK cells, and CD56^dim NK cells from one representative patient at the acute phase of infection. f Comparison of Bcl-2 expression using the median fluorescence intensity ratio (MFI, ratio of Ki67⁺ normalized to Ki67⁻) within T cells, CD56^bright NK cells, and CD56^dim NK cells (n = 9). Lowest and highest observations are displayed with the median indicated as center line. Statistical differences in f were tested using one-way analysis of variance (ANOVA) followed by Holm–Sidak’s multiple comparison test. *P < 0.05 and ***p < 0.001. Source data are provided as a Source Data file