Fig. 7
GSNOR function for Fe toxicity tolerance is conserved among higher plants. a Schematic representation of the gene structures and alleles of GSNOR from Lotus japonicus and rice. Red triangles indicate insert positions of LORE1 retrotransposon. Blue triangle indicates the position of guide RNA (gRNA) for CRISPR/Cas9. b, c Root growth of CRISPR/Cas9 knockout lines of OsGSNOR and WT. Upper panels, the roots before cutting; lower panels, the roots dispersed in water for total root length analysis. Scale bars: 2 cm. Quantified total root length is shown in box plots c. N, biological replicates. The percentage indicates the difference caused by high Fe. Different letters indicated the significant differences by one-way ANOVA with Tukey’s HSD test (p < 0.05). d Root growth at 50 µM Fe (control) and 350 µM Fe (high Fe) for 7 days. Scale bars: 5 mm. e Mean primary root length. Error bars: standard deviation. n = 8. Asterisk * and ** indicate the significant difference between WT and mutant lines at p < 0.05 and p < 0.01, respectively (Student’s t-test). The source data of Fig. 7c and e are provided in a Source Data file
