Fig. 3

Surviving Dbx1-derived OPCs become differentiated OLs. a–c Densities of YFP⁺/Olig2⁺ cells (a), YFP⁺/Olig2⁺/CC1⁻OPCs (b) and YFP⁺/Olig2⁺/CC1⁺ OLs (c) at PN4, PN10, PN19 and PN90 in the somatosensory cortex of Dbx1^CRE;Rosa26^YFP mice (dots represent n = 3 to 5 animals per age). Note that YFP⁺/Olig2⁺ cell density highly decreases from PN4 to PN10 (a). A reduction of YFP⁺/Olig2⁺/CC1⁻OPCs (b) is followed by an increase in YFP⁺/Olig2⁺/CC1⁺ OLs (c) during development (one-way ANOVA test followed by a Tukey’s Multiple Comparison test). d Confocal image of differentiated firstOLs at PN19 identified by the expression of YFP (green), Olig2 (red) and CC1 (magenta). Note the presence of a YFP⁺/Olig2⁻/CC1⁻interneuron in close vicinity of this YFP⁺/Olig2⁺/CC1⁺ OLs (white arrowhead). Scale bar: 20 µm. e–g Densities of total Olig2⁺ cells (e), Olig2⁺/CC1⁻OPCs (f) and Olig2⁺/CC1⁺ OLs (g) at PN4, PN10, PN19 and PN90 in the somatosensory cortex of Dbx1^CRE;Rosa26^YFP mice (dots represent n = 3 to 5 animals per age). Note that Olig2⁺ cell density decreases more from PN4 to PN10 than at later stages (e). A reduction of Olig2⁺/CC1⁻ OPCs (f) is followed by an increase in Olig2⁺/CC1⁺ OLs (g) during development (one-way ANOVA test followed by a Tukey’s Multiple Comparison test). Data are presented as mean ± SEM