Fig. 4

Cooperativity of EGR1 and TET1 modulate the enhancer activity of EGR1 binding sites. a Luciferase reporter assays for the control vector pCpGL-P and constructs with Galnt9, Npas4 locus. Fold change was normalized to the control vector pCpGL-P. Luciferase reporter assays for unmethylated or methylated Galnt9 (b) and Npas4 (c) constructs under either Egr1/Tet1 singularly or co-expression in primary cortical neurons. In figure b–c, fold changes were normalized to the methylated vectors without Egr1/Tet1 overexpression. Luciferase activity was measured at 48 h after transfection and normalized against the activity of a co-transfected firefly construct. mCpG represents methylated constructs. P-values were determined by t-test, *P < 0.05, **P < 0.01. Values represent mean ± s.d. from three biological replicates