Fig. 3
From: Genetic programming of macrophages to perform anti-tumor functions using targeted mRNA nanocarriers
IRF5/ IKKβ mRNA nanoparticles can imprint a pro-inflammatory M1-like phenotype. a Timelines depicting NP transfection protocols and culture conditions for the BMDMs used in panels (b–d). b Gene expression profiles of IRF5/IKKβ NP-transfected macrophages compared to signature M1 cells stimulated with the Toll-like Receptor 6 agonist MPLA. Results are depicted as a Volcano plot that shows the distribution of the fold changes in gene expression. M1 signature genes are indicated. The P value of overlap between IRF5/IKKβ NP-transfected macrophages and the M1 signature gene set was determined by GSEA. c Heat map of M1 signature gene expression in macrophages cultured in IL-4 versus cells cultured in IL-4 and transfected with IRF5/IKKβ NPs. d Box plots showing counts for indicated genes. The boxes represent the mean values and the line in the box represents median. The bars across the boxes show the minimum and maximum values. Whiskers represent 95% confidence intervals. N = 5 biologically independent samples
