Fig. 8
AMPK inhibits the transport pathways through ALDH7A1. Quantitative results are shown as mean with standard deviation; *p < 0.05, NS not significant, paired two-tailed Student’s t-test; n = 3 independent experiments. Source data are provided as a Source Data file. a COPI transport from the Golgi to the ER in HeLa cells was assessed through the quantitative colocalization of VSVG-KDELR with a cis-Golgi marker (giantin). b Fluid-phase endocytosis of dextran in HeLa cells was assessed through the quantitative colocalization of internalized dextran with an early endosome marker (EEA1). c COPI transport from the Golgi to the ER in HeLa cells was assessed through the quantitative colocalization of VSVG-KDELR with a cis-Golgi marker (giantin). d Fluid-phase endocytosis of dextran in HeLa cells was assessed through the quantitative colocalization of internalized dextran with an early endosome marker (EEA1). e COPI transport from the Golgi to the ER in HeLa cells was assessed through the quantitative colocalization of VSVG-KDELR with a cis-Golgi marker (giantin). f Fluid-phase endocytosis of dextran in HeLa cells was assessed through the quantitative colocalization of internalized dextran with an early endosome marker (EEA1). g HeLa cells were treated as indicated, and then Sirt1 activity was quantified. h EGF endocytosis in HeLa cells was assessed through the quantitative colocalization of internalized EGF with an early endosome marker (EEA1). i COPI transport from the Golgi to the ER in HeLa cells was assessed through the quantitative colocalization of VSVG-KDELR with a cis-Golgi marker (giantin). j Fluid-phase endocytosis of dextran in HeLa cells was assessed through the quantitative colocalization of internalized dextran with an early endosome marker (EEA1)
