Fig. 2

Associations between environmental temperature, physiology, and molecular substitution rates of lacertids. a Time-calibrated phylogeny derived from partitioned ML analysis of the combined dataset for 262 lacertid species. Colors represent character state reconstruction for yearly hours >30 °C of the species’ ranges computed from an occurrence record database. b–d Scatterplots among Tpref and IWL, and root-to-tip path (a proxy for molecular substitution rate) against a bioclimatic variable (yearly hours >30 °C) derived from distribution range information (all phylogenetic regressions significant; fitted regression lines calculated using a phylogenetic linear model, see main text and test statistics in Supplementary Information). e Plot of R2 vs. slope clues for regressions of root-to-tip paths vs. environmental temperature for 5878 gene trees derived from the RNAseq analysis, showing a positive slope for the majority of genes. Black dots are statistically significant correlations at P < 0.05. Source data are provided as a Source Data file