Fig. 1
AT-1S113R/+ show spontaneous steatosis while AT-1 sTg are resistant to diet-induced steatosis. a Schematic representation of reduced acetyl-CoA transport through AT-1 in the haploinsufficient AT-1S113R/+ mice. b Representative H&E staining of liver sections from WT and AT-1S113R/+ mice. Scale bar, 250 µm (left), 100 µm (right). c Representative electron microscopy of the liver sections from WT and AT-1S113R/+ mice. Scale bar, 5 µm. d Food intake of AT-1S113R/+ (n = 6) mice and age-matched WT (n = 7) littermates. e Body weight of 6-month-old AT-1S113R/+ (n = 31) mice and age-matched WT (n = 23) littermates. f Schematic representation of increased acetyl-CoA transport through AT-1 in systemic overexpression AT-1 sTg mice. g Representative H&E staining of the liver sections from WT and AT-1 sTg fed a high-carb diet (HCD). Scale bar, 250 µm (left), 100 µm (right). h LipidTOX green staining of the liver sections of WT and AT-1 sTg mice fed a HCD. Representative images (left panel) and quantification (right panel) are shown (WT, n = 3; AT-1 sTg, n = 3). Scale bar, 25 µm. i Food intake of AT-1 sTg (n = 6) and WT (n = 7) littermates while fed a high-carb diet. j Body weight of 3-month-old AT-1 sTg (n = 8) mice and age-matched WT (n = 8) littermates at week 0 and week 4 of the high-carb diet. k Fatty acid oxidative (FAO) capacity as examined by Seahorse assay in primary cultured hepatocytes (hepatocytes from biologically independent animals; WT, n = 4; AT-1 sTg, n = 4; AT-1S113R/+, n = 4). Statistical analysis was performed using a one-tailed Student’s t test. *P < 0.05; **P < 0.005; #P < 0.0005; All data are represented as mean ± s.d. Circles indicate individual animals
