Fig. 2

AT-1 sTg and AT-1^S113R/+ display proteomic changes across many metabolic pathways. a Volcano plot displaying all quantified proteins in AT-1 sTg (n = 4; left) and AT-1^S113R/+ (n = 4; right) livers, compared with age-matched WT littermates. Statistically significant proteins (2056 in AT-1 sTg; 373 in AT-1^S113R/+) are highlighted in yellow, and all other proteins are designated in blue. Fisher’s method, P < 0.05. b Histogram and box and whiskers plot showing the distribution of all proteins’ log₂ fold change in AT-1 sTg and AT-1^S113R/+, as compared with WT. The data represented as box and whisker plots: box represents the 25th to 75th inner quartile range, with the middle line denoting the median, and the inner square denoting the mean; the whiskers represent the interquartile distance with a coefficient of 1.5; the x represents the 1st and 99th percentile. Kolmogorov–Smirnov test. ^#P < 0.0005. c Venn diagram displaying significant protein overlap within models of AT-1 dysregulation. All proteins shown in panel a (yellow) were analyzed. d Heat-map showing the expression profile of the 61 overlapping proteins found in both AT-1 sTg and AT-1^S113R/+. Subcellular localization was determined according to the Uniprot database annotation. Secretory pathway includes the endoplasmic reticulum, Golgi apparatus, proteasome, lysosome, extracellular, and secreted proteins. Cytoplasm includes cytoskeleton, cytosol, ribosomes, and all other organelles. e Venn diagram displaying KEGG pathways enriched in the significant proteins within models of AT-1 dysregulation. Parameters for enrichment can be found in the proteomics methods. f The fold enrichment of KEGG pathways were organized by key cellular processes within models of AT-1 dysregulation. The original data set is shown in Supplementary Data 1. All proteins analyzed in c–f were significantly different (P < 0.05, Fisher’s method) from age-matched WT littermates