Fig. 3
AT-1 sTg and AT-1S113R/+ mice show changes in stoichiometry of lysine acetylation across many metabolic pathways. a Volcano plot of percent change in lysine acetylation sites detected in AT-1 sTg (n = 4; left) and AT-1S113R/+ (n = 4; right) mice, compared with age-matched WT littermates. Statistically significant sites (375 in AT-1 sTg; 415 in AT-1S113R/+) are highlighted in yellow, and all other detected sites are designated in blue. One-way ANOVA, P < 0.05. b Percent change of lysine site stoichiometry of all detected lysine sites in both models of AT-1 dysregulation. The data represented as box and whisker plots: box represents 25th to 75th inner quartile range; middle line denoting the median, and the inner square denoting the mean; whiskers represent the interquartile distance with a coefficient of 1.5; the most extreme values are the minimum and maximum, and the other dots are the 99th percentile and 1st percentile. Kolmogorov–Smirnov test. #P < 0.0005. c The number of acetylation sites affected per protein. d Venn diagram showing the overlap between significant lysine sites (upper) and the overlap between acetylated proteins (lower) in AT-1 sTg and AT-1S113R/+ mice. e Venn diagram of enriched KEGG pathways, and their enrichment scores found in models of AT-1 dysregulation. Purple denotes AT-1 sTg, and orange denotes AT-1S113R/+. Parameters for enrichment can be found in the Methods section. f Heatmap showing the percent change in stoichiometry of the 88 overlapping sites found in both models. Subcellular localization was determined according to the Uniprot database annotation. The secretory pathway includes the endoplasmic reticulum, Golgi apparatus, proteasome, lysosome, extracellular, and secreted proteins. Cytoplasm includes the cytoskeleton, cytosol, ribosomes, and all other organelles. g, h The distribution of significant lysine acetylation sites according to their Uniprot subcellular annotation, as a global view in AT-1 sTg (upper) and AT-1S113R/+ (lower) mice (g), and arranged by localization: the secretory pathway (purple circles), mitochondria (yellow circles), nucleus (blue circles), and cytosol (orange circles) (h). The original data set is shown in Supplementary Data 2. All sites analyzed in d–h were significantly different (P < 0.05, one-way ANOVA) from age-matched WT littermates. Bars are mean ± s.d. Circles indicate the average value of each acetylation site
