Fig. 4

AT-1 sTg and AT-1^S113R/+ mice show significant metabolic and mitochondria adaptation. a Venn diagram of overlapping significant proteins found to be modified in their expression levels (proteome) and in their stoichiometry of lysine acetylation (acetylome) within AT-1 sTg (upper) and AT-1^S113R/+ (lower) livers. b, c Proteins found to be enriched in lipid metabolism (b) and mitochondria-related (c) KEGG pathways are shown in clusters according to their level of regulation. Changes in the stoichiometry of acetylation are indicated by a purple (AT-1 sTg) or orange (AT-1^S113R/+) acetyl group; changes in the proteome are indicated by a purple (AT-1 sTg) or orange (AT-1^S113R/+) circle; no change in proteome level is indicated by a gray circle. d, e Mitochondria morphology in primary-cultured hepatocytes was examined with CellLight mitochondria stain (scale bar, 1 µm) (d) and quantified using Imaris reconstruction of area and volume (e) (hepatocytes from biologically independent animals; WT, n = 6; AT-1 sTg, n = 7 ; AT-1^S113R/+, n = 7). One-tailed Student's t test. **P < 0.005; ^#P <0.0005. f mRNA levels of PGC1α (pan) in liver samples (livers from biologically independent animals; WT, n = 5; AT-1 sTg, n = 5; AT-1^S113R/+, n = 5). One-tailed Student's t test. **P < 0.005. g Primary cultured hepatocytes were labeled with ¹³C-glutamine for 30 min before collection. The total TCA intermediate metabolite levels and ¹³C-labeled metabolite levels are shown (hepatocytes from biologically independent animals; WT, n = 5; AT-1 sTg, n = 6; AT-1^S113R/+, n = 5). Two-tailed Student’s t test. *P < 0.05. All data are represented as mean ± s.d.