Fig. 2 | Nature Communications

Fig. 2

From: Cryo-EM structures of lipopolysaccharide transporter LptB2FGC in lipopolysaccharide or AMP-PNP-bound states reveal its transport mechanism

Fig. 2

sfLptB2FG recognition of LPS. a Residues of LptF and LptG from the cavity that interact with LPS. LPS is shown in sphere and grey, whereas residues of LptF and LptG are shown in cyan and magenta, respectively. b 180° rotation of a along y-axis. c Functional assays of LptG residues. Mutants R136E, F67E/Y320E, Y257E/Y271E and K34E are lethal, whereas mutants R133E, K62E and L26E/M70E do not affect the bacterial growth. d Expression level of LptF and LptG of the mutants. The western blot showed that the mutant protein expression levels are similar to that of the wild type. Source data for panel d are provided as a Source Data file

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