Fig. 2

Epitope binning of human anti-Pfs25 antibodies. a Surface representation of Pfs25 showing the two previously characterized epitopes: Site 1 (1269, green) and Site 2 (1260, purple), and the C-terminus (C-Term) where Pfs25 was fused to the VLP. b Previously characterized Fabs are listed along the top and are the primary Fabs in the BLI competition assay. Secondary (competing) human Fabs are to the left. Reported scores are a percentage of total potential binding of that antibody, and therefore higher numbers (blue) display low amounts of competition, while low numbers (red) exhibit greater competition. Any experiment with >100% binding was given a score of 100, while negative values were given a score of 0. Human antibodies that fit previously defined epitopes Site 1 and Site 2 are outlined in green and purple, respectively. Potential novel epitope bins are highlighted by a dashed-line box