Fig. 4
From: Roles for DNA polymerase δ in initiating and terminating leading strand DNA replication
Exceptions to a canonical division of labor are conserved in S. pombe. Data are an average of at least three replicates for each genotype. a Fractional Pol ε synthesis (fPol ε) of top (blue) and bottom (yellow) strands. Points represent 50 bp bins. Curves are 2 kb moving averages. b, c The DDAF profile (orange points): the total fraction of synthesis by Pols α and δ across both strands. Green bars represent origin positions. Green diamonds indicate origin efficiencies over 0.4. Red bars indicate predicted collision positions assuming uniform global fork rates, uniform firing times and 100% origin efficiency. Points represent 50 bp bins. Curves are 3 kb moving averages. b DDAF peaks at four relatively efficient replication origins. Curves are 1.5 kb moving averages. c The DDAF across a section of Chromosome 2. Curves are 4 kb moving averages. Non-unique regions are excluded (purple). d A DDAF heatmap in 50 bp bins (3-bin moving average) for 1.2 kb on either side of 283 replication origins (green bars in a). Blue indicates a high DDAF (less Pol ε usage), red denotes the opposite. e Averaged DDAF curve at inefficient (efficiency < 0.5). The number of origins used is indicated below the graph. f Averaged DDAF peak at efficient (>0.7) origins. g DDAF peak areas (after baseline subtraction) increase linearly with origin efficiency (R2 = 0.990). h Early firing origin42 pairs (efficiencies > 0.2, intervening origin efficiency < 0.1, separation > 20 kb) have broad DDAF peaks centered near the inter-origin midpoint (orange curve; truncated at member origins). These peaks resemble collision point-centered S. cerevisiae DDAF peaks (brown dotted curve). i Comparison of tract length inferred from DDAF peak area in S. cerevisiae and S. pombe
