Fig. 5

TGFβ expression is associated with pSmad2/3 in Spont-PyMT tumors. a mRNA levels of Tgfβ were measured in untreated Spont-PyMT (gray diamonds) and Trans-PyMT (open circles) tumors, right after tumor dissociation. The relative expression, normalized to Trans-PyMT tumors, is shown. Each dot corresponds to one tumor. Cumulative data from Trans-PyMT mice: n = 10 and Spont-PyMT mice: n = 3. Student's t test. *p < 0.05; **p < 0.01; ***p < 0.001. b Tumors slices from Spont- and Trans-PyMT-untreated mice were stained with anti-EpCAM (blue) and anti-pSmad2/3 (red) Abs. c In Spont-PyMT tumors, pSmad2/3 ⁺ cells (red) may be myeloid cells (F4/80⁺, green), fibroblasts (gp38⁺, yellow), or tumor cells (EpCAM^+, blue). Note that the 2nd and 3rd images, not superimposed for clarity, are from the same image field. Images are representative of Spont-PyMT: n = 3 mice; Trans-PyMT: n = 3 mice, from three independent experiments for each group of mice. In b and c, scale bars = 50 µm. Source data are provided as a Source Data file