Fig. 4

Effect of nicotine on BAT and WAT in KOR null mice. a–c Representative images (left panels) and protein levels of UCP1 in the BAT (right panels) (WT vehicle n = 11, WT nicotine n = 12, KO vehicle n = 8, KO nicotine n = 8 mice). b–d mRNA levels of thermogenic markers in the BAT (WT vehicle n = 8, 8, 8, 8, 8, 7, WT nicotine n = 8, KO vehicle n = 8, KO nicotine n = 8, 8, 8, 7, 8, 7 mice). e–f UCP1 staining (left panels 40×, scale bar: 100 μm) and UCP1 stained area (right panels) in gWAT (WT vehicle n = 10, WT nicotine n = 12, KO vehicle n = 14, KO nicotine n = 11 mice). g–h H&E staining (left panels 40×, scale bar: 100 μm) and adipocyte area (right panels) in gWAT (WT vehicle n = 10, WT nicotine n = 13, KO vehicle n = 14, KO nicotine n = 11 mice). i–j mRNA levels of thermogenic markers in the gWAT (WT vehicle n = 7, 8, 7, 7, WT nicotine n = 7, 7, 6, 8, KO vehicle n = 6, 8, 7, 8, KO nicotine n = 8, 8, 8, 8 mice) of WT (a, b, e, g and i) or KOR KO (c, d, f, h and j) mice treated with vehicle or nicotine. Center values represent average; error bars represent SEM. Statistical significance was determined by two-sided t-Student; *P < 0.05, **P < 0.01, ***P < 0.001 vs. vehicle. The experiments were repeated two times; the samples represent biological replicates. In the panels a and c showing images of gels, all the bands for each picture come from the same gel but they have been spliced for clarity. Source data are provided as a Source Data file