Fig. 3
From: Retroviral integration into nucleosomes through DNA looping and sliding along the histone octamer
Asymmetric reconstruction of the isolated D02 NCP. a Streptavidin labelling allows asymmetric reconstruction of the D02 NCP, which avoids pseudo-two-fold symmetry averaging of the integration-site DNA. b 2D averages of labelled D02 NCP particles reveal a discernible, diffuse density for streptavidin at the nucleosomal DNA arm distal from the integration site. c 3D reconstruction of D02 NCP reveals an asymmetric streptavidin label decorating one of two nucleosomal DNA arms. d Unlike for Widom 601, the D02 NCP contains limited density for the N-terminal H2A tail, indicating that this element is flexible and available for intasome engagement. In fact, in the Intasome-NCP strand-transfer complex the N-terminal H2A tail interacts with IN, stabilising the interaction (also see Fig. 2b, c)
