Fig. 2 | Nature Communications

Fig. 2

From: Bacterial co-culture with cell signaling translator and growth controller modules for autonomously regulated culture composition

Fig. 2

AI-1 quorum sensing signal controlled growth rate through expression of HPr. a Schematic of AI-1 growth responsive controller cells (PH04 pAHL-HPr). AI-1 binds LasR (constitutively expressed) and activates the las promoter resulting in expression of HPr which increases cell growth rate. b Growth curves of PH04 pAHL-HPr cultures grown with varying levels of AI-1. Cultures were grown to OD 0.15 (t = 0) and AI-1 was supplemented at 40 min (indicated by arrow). Table shows the average growth rate from 1 to 4 h after AI-1 addition. c Growth rate relative to basal growth rate (either 0.28 or 0.32 h−1) for different AI-1 concentrations is plotted for two separate experiments (yellow and orange dots). Function fHPr describing relative growth rate as a function of AI-1 is plotted (blue line). A = 1, B = 1, C = 29, D = 2.1, E = 0.48. Source data are provided as a Source Data file

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