Fig. 6
From: Recurrent GNAQ mutation encoding T96S in natural killer/T cell lymphoma
Gαq suppresses AKT and MAPK signaling pathways in NK cells. a RNA sequencing and subsequent gene set enrichment analysis (GSEA) were performed on NKYS cells stably expressing vector control or wild-type Gαq. The P value of GSEA was computed by a 1000-gene-set two-sided permutation test. b p-ERK and p-AKT expression in NK cells from Ncr1-Cre-Gnaqfl/fl and Gnaqfl/fl mice. c The effect of wild-type Gαq or T96S mutant on the activation of ERK and AKT in YT (left) and KHYG1 (right) cells. GAPDH was used as a loading control. d Representative immunostaining of p-ERK and p-AKT in the xenograft tumor tissues; scale bars, 50 μm. e Immunohistochemical study of p-ERK and p-AKT in human NKTCL samples with wild-type Gαq (n = 90) and T96S mutant (n = 9). The expression levels of p-ERK and p-AKT were scored using the semiquantitative H-score (histochemical score) visual approach, taking into consideration the intensity of staining and the percentage of stained cells. All data are expressed as the mean ± s.e.m.; NS, not significant. *P < 0.05, **P < 0.01, ***P < 0.001, unpaired two-tailed Student’s t-test. Source data are provided as a Source Data file
