Fig. 1
qE capacity in P. tricornutum Lhcx1 mutants. a Western blot of low light grown strains, from left to right: wild type, three x1KO lines (x1KO_1a,1b and 2), two complemented lines of the x1KO_1a with the Lhcx1 gene cloned between the Lhcx1 promoter and terminator (x1KO + x1a,b) and between the FcpA promoter (x1KO + Fcp_x1a,b), respectively, and one complemented line of the x1KO_2 line (x1KO_2 + x1). After blotting, the blot was cut and the upper part was incubated with a Rubisco antibody, whereas the lower part was incubated with the Lhcx antibody. Protein mass marker bands are indicated on the left. b NPQ development during 3 min of actinic high light (white bar) followed by 6 min of recovery in darkness (black bar). Representative traces are depicted. c Mean doubling time in the exponential growth phase in the wild type and three x1KO lines during low light growth. SE is given. Statistically significant differences between the x1KO lines and the wild-type are indicated by a * (unpaired t test, eight degrees of freedom, p < 0.05, n = 5 biological replicates). Source data are provided as a Source Data file
