Fig. 9
From: CircAnks1a in the spinal cord regulates hypersensitivity in a rodent model of neuropathic pain
CircAnks1a interacted with miR-324-3p. a RIP showed that circAnks1a was enriched in AGO2 immunoprecipitates (**P < 0.01 vs. IgG group, two-tailed two-sample t tests, n = 4). b 9 miRNAs had at least 3 potential sites in circAnks1a by in silico analysis. c A luciferase reporter assay for the luciferase activity of Luc-circAnks1a in 293T cells transfected with different miRNA mimics was used to identify miRNAs that bound to the circAnks1a sequence (n = 6). d Compared with the scramble probe, the level of miR-324-3p pulled-down by the circAnks1a probe was obviously higher (**P < 0.01 vs. scramble group, two-tailed two-sample t tests, n = 4). e MiR-324-3p probe was used to examine the interaction between miR-324-3p and circAnks1a in dorsal horn tissues (**P < 0.01 vs. miR control group, two-tailed two-sample t tests, n = 4). f, g FISH showed that miR-324-3p was expressed in neurons and that it colocalized with circAnks1a-positive cells. Scale bars, 50 and 10 μm, respectively (n = 3). The data are presented as the mean ± s.e.m. Source data is available as a Source Data file
