Fig. 6 | Nature Communications

Fig. 6

From: Human induced pluripotent stem cell-derived vocal fold mucosa mimics development and responses to smoke exposure

Fig. 6

Pattern of expression of stratified markers in hiPSC-derived VFEC. a, b Hematoxylin–eosin staining showing differences in morphology between hiPSC-derived VFEC (a) and human native VF mucosa (b). A solid black arrow denotes a flattened luminal cell layer. c–h Double immunofluorescent staining showing expression of Cytokeratin 14 (green) and p63 gene (red) in hiPSC-derived VFEC (c, e) and native VF mucosa (f, h) and anti-p63 staining showing p63 + basal cells in hiPSC-derived VFEC (d) and native VF mucosa (g). i–l Double immunofluorescent staining showing the expression of Cytokeratin 13 (green) along with p63 (red) in hiPSC-derived VFEC (i, k) and native VF mucosa (l). j Anti-p63 staining showing p63 + basal cells in hiPSC-derived VFEC. m, n Immunofluorescent staining showing pattern of expression of Cytokeratin 8 in hiPSC-derived VFEC (m) and human native VF mucosa (n). Scale bar = 200 μm. hiPSC human induced pluripotent stem cell, VF vocal fold

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