Fig. 3
From: MLL1 is required for PAX7 expression and satellite cell self-renewal in mice
Loss of MLL1 abolishes PAX7 expression and myoblast proliferation, but does not affect myogenic differentiation. a Immunostaining of PAX7 (green) and Ki67 (red) in control and Mll1 cKO primary myoblasts. Nuclei are counterstained with DAPI (blue). Scale bars represent 20 µm. b Quantification of PAX7-expressing cells from control and Mll1 cKO primary myoblasts. Data are presented as the mean ± S.E.M. of three independent experiments. c Western blot analysis of PAX7 and MYOD in control and Mll1 cKO primary myoblasts. GAPDH is used as a loading control. d Densitometric analysis of the relative levels of PAX7 normalized to GAPDH signals of six independent experiments. Values are represented as the mean ± S.E.M. e Control and Mll1 cKO primary myoblasts were plated at the same density and grown for 6 days. Every 2 days, cells were numerated to evaluate their proliferation, represented as the mean (n = 3 biological replicates) ± S.E.M. f Quantification of Ki67-expressing cells from control and Mll1 cKO primary myoblasts. Data are presented as the mean (n = 3 biological replicates) ± S.E.M. g Western blot analysis of Myosin Heavy Chains (MHC) and MYOGENIN during differentiation of control and Mll1 cKO primary myoblasts. TUBULIN is used as a loading control. h Immunostaining of MHC (green) and PAX7 (red) in control and Mll1 cKO primary myoblasts cultured in differentiation medium for 6 days. Nuclei are counterstained with DAPI (blue). Scale bar represents 100 µm
