Table 1 Partial list of genes differentially expressed in Mll1 cKO and control primary myoblasts
From: MLL1 is required for PAX7 expression and satellite cell self-renewal in mice
Gene symbol | Array signal Control | Array signal Mll1 cKO | Array Fold change | qPCR Fold change | |
|---|---|---|---|---|---|
Downregulated genes | |||||
1 | Myf5 | 9.51 | 6.14 | −10.33 | −20.00 |
2 | Asb4 | 9.99 | 7.12 | −7.31 | −14.28 |
3 | Six2 | 9.20 | 7.67 | −2.89 | −7.14 |
4 | Dlx1 | 9.00 | 7.74 | −2.40 | −2.85 |
5 | Fgfr4 | 9.00 | 7.77 | −2.34 | −2.43 |
6 | Olfr767 | 7.64 | 6.46 | −2.27 | nd |
7 | Lbx1 | 7.97 | 6.82 | −2.22 | −5.26 |
8 | Lmnb1 | 8.91 | 7.84 | −2.11 | nd |
9 | Cdc42ep3 | 8.69 | 7.64 | −2.07 | nd |
10 | Lbr | 9.04 | 8.00 | −2.05 | nd |
Upregulated genes | |||||
1 | Slc40a1 | 5.84 | 8.87 | 8.16 | nd |
2 | Timp3 | 5.60 | 8.59 | 7.94 | nd |
3 | Stmn2 | 5.02 | 7.89 | 7.33 | nd |
4 | Dlk1 | 6.70 | 9.37 | 6.37 | 18.25 |
5 | Sfrp2 | 6.41 | 8.95 | 5.83 | 4.64 |
6 | Cd24a | 7.97 | 10.50 | 5.76 | 4.96 |
7 | Nnat | 7.15 | 9.40 | 4.75 | nd |
8 | RbEST47 | 6.75 | 8.95 | 4.62 | nd |
9 | Ly6a (Sca1) | 6.55 | 8.73 | 4.54 | 2.89 |
10 | Krt18 | 6.19 | 8.26 | 4.22 | nd |
Genes related to myogenesis | |||||
1 | Myod1 | 12.15 | 12.27 | 1.08 | 1.33 |
2 | Myogenin | 7.30 | 7.94 | 1.56 | 1.17 |
3 | Myf6 | 5.40 | 5.07 | −1.26 | −1.70 |
4 | Mef2a | 11.08 | 11.12 | 1.03 | nd |
5 | Mef2b | 6.44 | 6.42 | −1.01 | nd |
6 | Mef2c | 6.25 | 7.69 | 2.71 | nd |
7 | Myh1 | 6.62 | 7.86 | 2.36 | nd |
8 | Pax3 | 6.44 | 6.99 | 1.47 | nd |
9 | Pax7 | 10.29 | 10.02 | −1.21 | −1.58 |
MLL1 target genes | |||||
1 | Hoxa6 | 7.44 | 7.47 | 1.02 | nd |
2 | Hoxa7 | 7.64 | 7.88 | 1.18 | nd |
3 | Hoxa10 | 7.74 | 7.65 | −1.06 | nd |
4 | Hoxa11 | 8.28 | 7.81 | −1.38 | nd |
5 | Hoxc6 | 7.84 | 8.06 | 1.16 | nd |
6 | Hoxc8 | 8.03 | 7.59 | −1.36 | nd |
7 | Hoxc9 | 8.44 | 8.69 | 1.19 | nd |
8 | Hoxc10 | 8.51 | 8.65 | 1.10 | nd |
9 | Dlx2 | 10.44 | 10.79 | 1.27 | nd |
10 | Prdm16 | 6.41 | 5.85 | −1.47 | nd |
Histone methyltransferases | |||||
1 | Mll1 (Kmt2a) | 9.44 | 9.66 | 1.17 | nd |
2 | Mll2 (Kmt2b) | 7.90 | 8.05 | 1.11 | nd |
3 | Setd1a | 8.81 | 8.88 | 1.05 | nd |
4 | Setd1b | 8.52 | 8.42 | −1.07 | nd |
