Fig. 2

Feasibility of RIMRs. a Production amount of 3-PGA as a function of the reaction time for the RIMRs (solid red circles) and the BIMRs (open dark squares). The red dash-dotted line and dark dashed line are the liner fitting regressions. The slope of liner fitting regression of 3-PGA production in RIMRs is 13.8 μmol g⁻¹ RuBisCO min⁻¹ and that in BIMRs is almost 0. The initially injected RuBisCO and BSA concentrations for immobilization are both 6.25 μg μL⁻¹. The collected production solutions I from RIMRs and BIMRs are 21 μL. RuBP concentration is 0.5 mM. \({\text{HCO}}_{3}^{-}\) in the reaction buffer is 66 mM. Reaction temperature is 30 °C. b HPLC–MS/MS chromatography of RuBP and 3-PGA in the production solutions I obtained from RIMRs and BIMRs at the reaction time of 5 min. Error bars represent the standard deviations from three independent experiments. Source data are provided as a Source Data file