Fig. 2 | Nature Communications

Fig. 2

From: Selective autophagy maintains centrosome integrity and accurate mitosis by turnover of centriolar satellites

Fig. 2

Autophagy deficiency leads to chromosome segregation defects and postmitotic cell death. a Time-lapse imaging experiments of stable U2OS mRFP-α-tubulin H2B-GFP cells transfected with control, ULK1 or ATG7 siRNAs. GFP and DIC channels were recorded. Time-points after NEBD are indicated. b Quantification of phenotype distribution shown in (a). SCR, n = 169; ULK1, n = 47; ATG7 n = 179. c Representative immunofluorescence images of Hoechst33342-stained U2OS cells treated with control, ULK1, ATG7 or ATG5 siRNA for micronuclei quantification. Arrows indicate micronuclei. d Quantification of experiments shown in (c). Columns represent the mean ± SD, n = 3 ≥ 100 cells, **P ≤ 0.01, ***P ≤ 0.001. Unpaired Student’s t-test, two-tailed. Scale bars, 10 µm. Source data are provided as a Source Data file

Back to article page