Fig. 4
Centriolar satellite components interact with GABARAPs but not LC3B. a–c Scatter plots showing enrichment values (x-axis) and corresponding significance levels (y-axis) for proteins co-purifying with GFP-tagged GABARAP, GABARAPL2 or LC3B, n = 4. Centrosomal proteins are indicated in green, horizontal line indicates significance with threshold P-value < 0.05. d Heat map representing abundance of centrosomal proteins across co-IPs from A-C with p < 0.05 for at least one bait. Data are bait normalized to correct for differences in expression levels of bait proteins. e GFP-precipitation of lysates from inducible GFP-3xFLAG or GFP-tagged LC3B, GABARAP or GABARAPL2 MCF7 cells analyzed for co-precipitation of endogenous CEP131 and PCM1, n = 3. f GFP precipitation of GFP-3xFLAG, GFP-GABARAP or GFP-GABARAPL2 in U2OS cells treated with control, PCM1 or CEP131 siRNAs and blotted for co-precipitation of endogenous PCM1 and CEP131, n = 3. g GFP-precipitation of HEK293 lysates following co-transfection of GFP-3xFLAG or GFP-tagged GABARAP or GABARAPL2 with HA-PCM1-wt or HA-PCM1-3XA analyzed for co-precipitation of HA-tagged PCM1 variants, n = 3. h GFP-precipitation of GFP-3xFLAG or GFP-tagged LC3B, GABARAP or GABARAPL2 in HEK293 cells analyzed for co-precipitation of endogenous SSX2IP, OFD1 and CEP290, n = 3
