Fig. 8
PCM1 accumulation and mitotic abnormalities show GABARAP selectivity. a Immunoblot of U2OS cell extracts following depletion of LC3B, GABARAP and GABARAPL2 individually or in combination as indicated showing levels of PCM1. GAPDH is used as loading control. b Densitometric quantification of PCM1 levels relative to GAPDH, represented in (a). Columns represent the mean ± SD, n = 3, ns > 0.05, **P ≤ 0.01, ***P ≤ 0.001. Unpaired Student’s t-test, two-tailed. c Representative images of PCM1-stained CS in U2OS cells depleted of the denoted ATG8 proteins. d Representative images of mitotic abnormalities in U2OS cells stained for γ-tubulin, β-tubulin and Hoechst33342 following depletion of the denoted ATG8 proteins. e Quantification of phenotype distribution in (d). Columns represent the mean ± SD, n = 3 of ≥20 cells, ns P > 0.05, **P ≤ 0.01. Unpaired Student’s t-test, two-tailed. Abnormal refers to cells exhibiting multipolar, diffuse bipolar or monopolar mitoses. Scale bars, 10 µm. Source data are provided as a Source Data file
