Fig. 8

Glu-lOLP is required for dark-induced pausing behavior. a, b Genetic manipulations of glu-lOLP affect dark-induced pausing behavior in larvae. a Plots of average pause frequency are shown. The transition from light to dark is indicated by the shade of the area. b Quantification of dark-induced pause frequency reveals the critical role of Rh6-PRs and glu-lOLP in this behavioral response. Statistical significance determined by one-way ANOVA: p < 2e − 16, F = 35.6, df = 7, 72 followed by post hoc Dunnetts’s multiple comparison’s test: lOLP^glu/+-lOLP^glu > rpr, hid: p < 1e − 04, t = 9.907; +/rpr,hid-lOLP^glu > rpr, hid: p < 1e − 04, t = 7.990; lOLP^glu/+- lOLP^glu > PTX: p < 1e − 04, t = 10.337; +/PTX-lOLP^glu > PTX: p = 0.000108, t = 4.648; lOLP^glu/+-lOLP^glu > mAChR-B^RNAi: p < 1e − 04, t = 7.120; +/mAChR-B^RNAi-lOLP^glu > mAChR-B^RNAi: p < 1e −04, t = 5.044. ***P < 0.001. n = 10 for each genotype. c, d The light-induced increase in turning frequency is reduced in Rh6 mutants but unaffected by glu-lOLP manipulations. c Plots of average turn frequency are shown. The transition from dark to light is indicated by the shade of the area. d Quantifications of the light-induced turn frequency reveals that glu-lOLP does not influence the behavioral responses induced by the dark to light transition. Statistical significance determined by one-way ANOVA: p < 7.16e − 12, F = 14.93, df = 7, 72 followed by post hoc Dunnetts’s multiple comparison’s test: lOLP^glu/+-lOLP^glu > rpr, hid: p = 0.753, t = −1.159; +/rpr,hid-lOLP^glu > rpr, hid: p = 0.538, t = 1.472; lOLP^glu/+-lOLP^glu > PTX: p = 0.530, t = 1.483; +/PTX-lOLP^glu > PTX: p = 0.996, t = 0.445; lOLP^glu/+-lOLP^glu > mAChR-B^RNAi: p < 0.001, t = −4.134; +/mAChR-B^RNAi-lOLP^glu > mAChR-B^RNAi: p = 0.849, t = −0.996. n.s., p > 0.05, ***p < 0.001. n = 10 for each genotype