Fig. 1
A quintuple mutant, bes1-1 bzr1-1 beh1-1 beh3-1 beh4-1 (qui-1), shows a male sterile phenotype due to shortened filaments and pollenless anthers. a, b Inflorescences phenotypes of wild-type Col-0 (a) and qui-1 (b). Col-0 siliques are elongated and produce viable seeds, while mutant siliques fail to elongate and are devoid of any seeds. c, d Optical micrographs of Col-0 (c) and qui-1 (d) flowers. qui-1 develops stamens with filaments much shorter than Col-0. e–h Anthers of Col-0 (e, g) and qui-1 (f, h) visualized by a SEM microscope (e, f) and Alexander staining (g, h), respectively. Col-0 anthers give rise to viable pollen grains while mutant anthers are completely pollenless. Scale bars represent 1 cm in (a), (b), 0.5 mm in (c), (d), and 50 μm in (e–h)
