Fig. 4

BES1-YFP and BZR1-YFP can be detected in the developing anthers. Seven-week-old pBES1::BES1-YFP (a–f) and pBZR1::BZR1-YFP (g–l) transgenic plants were used for confocal microscopic analyses. Stage 4–6 intact anthers were selected for analyzing the YFP signals. Green colors represent YFP signals and red colors are the auto-florescence of chlorophylls (a–c, g–i). To visualize detailed tissue-level localization of the YFP signal, somatic cell membranes were stained with FM4-64 (d–f, j–l). Inner secondary parietal cell layer (ISP) and tapetum (T) are indicated by arrow heads. Scale bars represent 20 μm