Fig. 5
bes1-1 bzr1-1 beh1-1 beh3-1 beh4-1 (qui-1), bes1-c1 bzr1-1 beh1-1 beh3-1 beh4-c1 (qui-2), tpd1, serk1 serk2, or ems1 show similar anther developmental defects. a Floral phenotypes of Col-0, qui-1, qui-2, tpd1, serk1/2, Ler and ems1. The qui-1 and qui-2 display shortened filaments similar to tpd1, serk1 serk2, and ems1. b, c SEM microscopic (b) and Alexander staining (c) images of the anthers from the plants corresponding to (a). qui-1 and qui-2 anthers cannot produce pollen grains, reminiscent to those of tpd1, serk1/2, and ems1. d Toluidine blue stained semithin sections of stage 6 anthers from plants corresponding to (a). All anthers of qui-1, qui-2, tpd1, serk1/2, and ems1 show excessive microsporocytes. qui-1 contains an impaired tapetal cell layer, whereas qui-2 only contains three somatic cell layers, lacking the tapetal cell layer, which is identical to those of tpd1, serk1/2, and ems1. Number of somatic cell layers are indicated by red asterisks. e Quantitative RT-PCR results showing relative expression levels of tapetum marker genes in the inflorescences of various mutants and their corresponding wild-type backgrounds. Data are presented as mean and s.d. (n = 3). Statistically significant differences between groups were tested using One-way ANOVA followed by LSD (least significant difference) post hoc test. Different letters indicate significant difference at P < 0.05. Scale bars represent 0.5 mm in (a), 50 μm in (b), (c), and 20 μm in (d)
