Fig. 7
From: TRIM66 reads unmodified H3R2K4 and H3K56ac to respond to DNA damage in embryonic stem cells
Loss of TRIM66 results in genomic instability in vivo. a Genotypic statistics of offspring mice. A total of 48 offspring after crossing 6 pairs of Trim66+/− mice. b Statistics of the body weight of mice offspring aged 2 or 3 weeks. c Representative immunofluorescence images of H3K56ac in blastocysts of WT Trim66+/+ and Trim66−/− mice. Scale bar, 10 μm. d Representative immunofluorescence images of γ-H2AX in WT and Trim66−/− blastocysts. Scale bar, 10 μm. e Immunoblot analysis of the levels of γ-H2AX and H3K56ac in the primary Trim66+/+ and Trim66−/− ESCs. f, g Representative immunofluorescence images of H3K56ac and γ-H2AX in the primary Trim66+/+ and Trim66−/− ESCs. Scale bar, 10 μm. h Quantification of chromosomal breakage (left) or chromosome ends fusion (right) in primary Trim66+/+ and Trim66−/− ESCs. More than 100 cells are examined in each sample. *represents a difference from Trim66+/+ mice or primary Trim66+/+ ESCs. Data are presented as the means ± SEM. Statistical significance is determined by two-tailed Student’s t-test. *p < 0.05, **p < 0.01, ***p < 0.001. Source data are provided as a Source Data file
