Fig. 3

Demonstration of high-speed live-cell imaging and instant image reconstruction. A typical survey of stained U2OS cells was carried out with our SIM system. Video-recording (full version in Supplementary Movie 1) of the real-time results as presented to the user (column a), and the SIM reconstructions of the 488 nm channel (column b), stained for mitochondria (MitoTracker Green), and of the 647 nm channel (column c), stained for the endoplasmic reticulum (ER-Tracker Red). Column d shows an overlay of both reconstructed channels. Imaging was performed in dual color at 10.4 fps with 2 ms illumination time per raw frame (see Fig. 2b), multiple cells were surveyed in quick succession (see timestamp in each row). SIM allows to clearly resolve mitochondria and the endoplasmic reticulum