Fig. 5

Demonstration of the system in use for live-cell imaging of mitochondrial dynamics. Single-color imaging of U2OS cells with 5 ms raw frame exposure time (see Fig. 2b), with overview (a) and time series of cut-out (b). The dynamics could be observed in real time and super-resolution by the system operator. The microscope hardware could easily provide higher speeds and continuous data acquisition, however, current dyes are limited in both brightness and photo-stability. Nonetheless, short exposure times are still very helpful in time-lapsed acquisition, as they suppress motion blur⁴⁶