Fig. 1 | Nature Communications

Fig. 1

From: Caspase-10 inhibits ATP-citrate lyase-mediated metabolic and epigenetic reprogramming to suppress tumorigenesis

Fig. 1The alternative text for this image may have been generated using AI.

Caspase-10 is upregulated upon metabolic stress a A549 cells were stably transfected (pooled puromycin-resistant population) with control (scrambled) or p53 shRNA. A549 control (A549) and p53 knockdown (A549 p53kd) cells were subjected to glucose starvation for the indicated time points. Relative mRNA levels were analyzed by RT-qPCR for the indicated genes. Error bars are means ± SD of three biological replicates. Statistical analyses were done using two-way ANOVA (Tukey’s post hoc test). *P < 0.05; **P < 0.01. b A549 control (A549) and p53 knockdown (A549 p53kd) cells were subjected to glucose starvation for the indicated time points. The cells were then harvested and western blotting was performed for indicated proteins. c A549 control (A549) and A549 p53 knockdown (A549 p53kd) cells were subjected to glucose starvation for the indicated time points. The cells were then harvested and caspase-10 activity was examined. Error bars are means ± SD of three biological replicates. Statistical analyses were done using two-way ANOVA (Tukey’s post hoc test). *P < 0.05; **P < 0.01. d, e A549 cells were stably transfected (pooled puromycin-resistant population) with control (LacZ) or the indicated shRNAs. Cells were subjected to unstressed (25 mM) or glucose starvation conditions (5 mM) for 36 h. The cells were then harvested and, (d) caspase-10 activity was examined or (e) western blotting was performed for the indicated proteins. Error bars are means ± SD of three biological replicates. Statistical analyses were done using two-way ANOVA (Bonferroni’s post hoc test). ***P < 0.001. f, g A549 cells were stably transfected with either empty vector or dominant negative mutant FADD-DN-FLAG construct. These cells were subjected to unstressed (25 mM) or glucose starvation conditions (5 mM) for 36 h. The cells were then harvested and (f) caspase-10 activity was examined or (g) western blotting was performed for the indicated proteins. Error bars are means ± SD of three biological replicates. Statistical analyses were done using two-way ANOVA (Bonferroni’s post hoc test). Source data are provided as a Source Data file

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