Fig. 3
Caspase-10 regulates nucleocytosolic acetyl-CoA levels a A549 cells were subjected to glucose starvation and concomitantly treated with DMSO (control) or caspase-10 inhibitor (Q-AEVD-FMK) (25 μM) for the indicated time points. The cells were then harvested and ACLY activity was examined. Error bars are means ± SD of three biological replicates. Statistical analyses were done using two-way ANOVA (Bonferroni’s post hoc test). **P < 0.01. b A549 control (A549) and caspase-10 knockdown (A549 CASP10kd) cells were subjected to glucose starvation for the indicated time points. The cells were then harvested and ACLY activity was examined. Error bars are means ± SD of three biological replicates. Statistical analyses were done using two-way ANOVA (Bonferroni’s post hoc test). **P < 0.01. c A549 ACLY knockdown cells (A549 ACLYkd) were stably transfected with wild-type ACLY (ACLY) or caspase-10-cleavage resistant mutant (ACLYD1026A). These cells were subjected to glucose starvation for the indicated time points followed by ACLY activity assay. Error bars are means ± SD of three biological replicates. Statistical analyses were done using two-way ANOVA (Bonferroni’s post hoc test). ***P < 0.001. d A549 control (A549) cells and A549 caspase-10 knockdown (A549 CASP10kd) cells were subjected to glucose starvation for the indicated time points. Nucleocytosolic fraction was then isolated followed by acetyl-CoA quantification. Error bars are means ± SD of three biological replicates. Statistical analyses were done using two-way ANOVA (Bonferroni’s post hoc test). ***P < 0.001. e A549 ACLY knockdown cells (A549 ACLYkd) were stably transfected with wild-type ACLY (ACLY) or caspase-10-cleavage resistant mutant (ACLYD1026A). These cells were subjected to glucose starvation for the indicated time points. Nucleocytosolic fraction was then isolated followed by acetyl-CoA quantification. Error bars are means ± SD of three biological replicates. Statistical analyses were done using two-way ANOVA (Bonferroni’s post hoc test). ***P < 0.001. Source data are provided as a Source Data file
