Fig. 4
Caspase-10 downregulates intracellular lipid levels and histone acetylation a–c A549 cells were stably transfected (pooled zeocin-resistant population) with control (scrambled), caspase-10 shRNA or caspase-10 shRNA along with ACLY shRNA. A549 control (A549), CASP10 knockdown (A549 CASP10kd) and CASP10/ACLY double knockdown (A549 CASP10kd/ACLYkd) cells were subjected to glucose starvation for the indicated time points followed by (a) free fatty acids quantification, (b) total triglycerides quantification, and (c) total cholesterol quantification. Error bars are means ± SD of three biological replicates. Statistical analyses were done using two-way ANOVA (Bonferroni’s post hoc test). ***P < 0.001. d A549 cells were subjected to glucose starvation for 48 h and treated with caspase-10 inhibitor, Q-AEVD-FMK (25 μM) or palmitate (0.2 mM) for last 24 h of starvation period. The cells were then stained with Nile Red (5 μg/ml) and examined. DAPI was used to counterstain the nucleus. Scale bar: 50 μm. e A549 control (A549), caspase-10 knockdown (A549 CASP10kd) and caspase-10/ACLY double knockdown (A549 CASP10kd/ACLYkd) cells were subjected to glucose starvation for the indicated time points. The cells were then harvested and western blotting was performed for the indicated proteins. f A549 cells were subjected to glucose starvation for 48 h and treated with sodium acetate (5 mM) for the last 24 h of starvation period. The cells were then harvested and western blotting was performed for the indicated proteins. g A549 ACLY knockdown cells (A549 ACLYkd) were stably transfected with wild-type ACLY (ACLY) or caspase-10-cleavage resistant mutant (ACLYD1026A). These cells were subjected to glucose starvation for the indicated time points. The cells were then harvested and western blotting was performed for the indicated proteins. Source data are provided as a Source Data file
