Fig. 5
Caspase-10 represses proliferative and metastatic genes a A549 control (control), caspase-10 knockdown (CASP10kd), caspase-10/GCN5 double knockdown (CASP10kd/GCN5kd), caspase-10/p300 double knockdown (CASP10kd/p300kd) and caspase-10/PCAF double knockdown (CASP10kd/PCAFkd) cells were subjected to glucose starvation for 48 h. The cells were then harvested and sandwich ELISA was performed for acetylated H3 and acetylated H4. Error bars are means ± SD of biological replicates. Statistical analyses were done using one-way ANOVA (Tukey’s post hoc test). **P < 0.01; ***P < 0.001. b A549 control (A549), caspase-10 knockdown (A549 CASP10kd) and caspase-10/GCN5 double knockdown (A549 CASP10kd/GCN5kd) cells were subjected to glucose starvation for the indicated time points. These cells were harvested and western blotting was performed for the indicated proteins. c A549 control (control), caspase-10 knockdown (CASP10kd), caspase-10/ACLY double knockdown (CASP10kd/ACLYkd) and caspase-10/GCN5 double knockdown (CASP10kd/GCN5kd) cells were subjected to glucose starvation for the indicated time points. Total RNA was then isolated and relative mRNA levels were analyzed by RT-qPCR for the indicated genes. Heat map comparing relative mRNA levels (mean fold change from three independent experiments with triplicate samples) is shown. Blue and red indicate upregulation or downregulation respectively. d A549 control (A549), caspase-10 knockdown (A549 CASP10kd), caspase-10/ACLY double knockdown (A549 CASP10kd/ACLYkd) and caspase-10/GCN5 double knockdown (A549 CASP10kd/GCN5kd) cells were subjected to glucose starvation for the indicated time points. ChIP assay was then performed with control IgG or acetylated-H3K9 antibody. Error bars are means ± SD of three biological replicates. Statistical analyses were done using two-way ANOVA (Tukey’s post hoc test). *P < 0.05; **P < 0.01; ***P < 0.001. Source data are provided as a Source Data file
