Fig. 3

Genome-wide CO landscape formation and feature association. a Sliding window-based (window size 1 Mb, step size 50 kb) recombination landscapes calculated for true positives (TP), false positives (FP) and the combined set of TP + FP recombinant molecules of P50L75. Heterochromatic regions⁴⁴ are indicated by rectangles in light blue. b Correlation tests of regional CO frequencies comparing TP vs. TP + FP (Pearson’s r 0.97), FP vs. TP + FP (Pearson’s r 0.25) and FP vs. TP (Pearson’s r 0.05). c Association of the TP and TP + FP sets with different genomic features in contrast to the random expectation (permutation test). d. Correlation tests of regional GC-content with CO frequency within the TP and TP + FP sets. e Association of TP or TP + FP sets with DNA methylation, compared with a random expectation (t-test; Methods). Note: in c and e, the expectations were obtained based on randomly sampled intervals within the reference genome excluding heterochromatic regions. The middle position of a CO interval was used for associations with genomic features in c, where the promoter of a gene was defined as the 1000 bp region upstream of the transcription start site, and the gene start/end as the first/last 200 bp of a gene. For each feature, a permutation test (oneway test in R coin package) was performed between the TP set (or TP + FP set) with 1,000 sets of randomly sampled 3000 intervals. The asterisks indicate the observed values (either from the TP or the TP + FP set) were significantly different from a random expectation. Source Data are provided as a Source Data file Source_Data_main_Figure_3.zip