Table 1 Effect of increasing the molecule number by increasing DNA loading

From: Linked-read sequencing of gametes allows efficient genome-wide analysis of meiotic recombination

Pool

P50L25

P50L40

P50L75

Raw molecules (≥1 kb)C

Raw molecules (≥1 kb)L

3,577,104

3,462,796

5,804,559

5,631,294

12,551,833

12,240,762

Filtered moleculesC

Filtered moleculesL

2,664,977

2,565,403

4,783,828

4,642,515

10,651,147

10,417,489

Total CO moleculesCL

558

1012

2519

TP CO moleculesCL

475

(85.1%)R

804

(79.4%)R

1874

(74.4%)R

TP unique (non-redundant) COsCL

254

(63.5%)U

325

(81.3%)U

363

(90.8%)U

FP moleculesCL

83

(14.9%)R

208

(20.6%)R

645

(25.6%)R

  1. TP true positive, FP false positive
  2. CNumber based on Col-0 reference genome38
  3. LNumber based on Ler reference genome39
  4. CLNumber based on intersection of two sets of CO predictions (of C and L)
  5. RPercent CO molecules of all molecules
  6. URecall rate