Table 1 Effect of increasing the molecule number by increasing DNA loading
From: Linked-read sequencing of gametes allows efficient genome-wide analysis of meiotic recombination
Pool | P50L25 | P50L40 | P50L75 |
|---|---|---|---|
Raw molecules (≥1 kb)C Raw molecules (≥1 kb)L | 3,577,104 3,462,796 | 5,804,559 5,631,294 | 12,551,833 12,240,762 |
Filtered moleculesC Filtered moleculesL | 2,664,977 2,565,403 | 4,783,828 4,642,515 | 10,651,147 10,417,489 |
Total CO moleculesCL | 558 | 1012 | 2519 |
TP CO moleculesCL | 475 (85.1%)R | 804 (79.4%)R | 1874 (74.4%)R |
TP unique (non-redundant) COsCL | 254 (63.5%)U | 325 (81.3%)U | 363 (90.8%)U |
FP moleculesCL | 83 (14.9%)R | 208 (20.6%)R | 645 (25.6%)R |