Fig. 3
From: Derailing the aspartate pathway of Mycobacterium tuberculosis to eradicate persistent infection
Endo and exometabolome of Mtb ∆thrA. a Starvation of the homoserine auxotroph Mtb ∆thrA in unsupplemented medium (red bars) leads to strong (up to 100-fold) intracellular accumulation of lysine and DAP and its biosynthetic intermediates, as well as downstream metabolites aminoadipate, and n-acetyl-lysine. Accumulation of the same intermediates is not observed in WT cells (Mtb H37Rv) under the same conditions (black bars). Addition of threonine to Mtb ∆thrA reverses accumulation of lysine and its intermediates (blue bars). Under the same conditions, aspartate but no other intermediate accumulates indicating that aspartate kinase is feedback inhibited by threonine. b Analysis of the exometabolome shows accumulation of lysine but no other intermediate in the supernatant of unsupplemented Mtb ∆thrA (red bars). Threonine addition stopped lysine export (blue bars). All values are the average of three biological replicates (n = 3) ±s.d. (error bars depict standard deviation) and are representative of a minimum of two independent experiments. **p-value < 0.01 in Student's t-test. Bars are overlaid with dot plot of individual values. Source data are provided as a Source Data file
