Fig. 4
From: Derailing the aspartate pathway of Mycobacterium tuberculosis to eradicate persistent infection
Threonine but not methionine deficiency leads to lysine overload. To confirm that threonine deficiency alone is responsible for accumulation of lysine and intermediates, the endo-metabolome of the threonine auxotroph Mtb ∆thrB was determined and compared to Mtb ∆thrA. Accumulation of DAP, lysine and n-acetyl-lysine was detected in both strains. Only threonine but not methionine reversed lysine accumulation in Mtb ∆thrA. All values are the average of three biological replicates (n = 3) ± s.d. (error bars depict standard deviation) and representative of two independent experiments. **p-value < 0.01 in Student's t-test
