Fig. 2
Short-term plasticity is shaped by calcium-dependent facilitation and release-independent depression. a, b Mean profiles of [DA]o transients evoked by single or paired electrical pulses in 1.2 mM (light grey), 2.4 mM (dark grey) and 3.6 mM (black) [Ca2+]o in CPu (a, n = 3) and NAc (b, n = 3). c Mean 1p-evoked [DA]o (normalised to 2.4 mM [Ca2+]o) vs [Ca2+]o in CPu (filled) and NAc (open circles). d, e Mean P2/P1 vs IPI in 1.2 mM (light grey), 2.4 mM (dark grey) and 3.6 mM (black) [Ca2+]o in CPu (d, filled) and NAc (d, open circles). f Mean P2/P1 vs mean peak 1p [DA]o at 10 ms IPI in 1.2 mM (light grey), 2.4 mM (dark grey) and 3.6 mM (black) [Ca2+]o in CPu (filled) and NAc (open circles). g Mean profiles of [DA]o transients elicited by single or paired light pulses at 40 ms IPI (473 nm, 2 ms) in 1.0 mM (light grey), 2.0 mM (dark grey) and 3.0 mM (black) [Ca2+]o in CPu (left, n = 3) and NAc (right, n = 3). Inset, representative voltammogram for optogenetically evoked DA release. h Mean peak 1p [DA]o (normalised to 2.4 mM [Ca2+]o) vs [Ca2+]o (log scale) evoked by light stimulation in CPu (filled) and NAc (open circles). Second-order polynomial curve fit; F-test of relative sum of squares shows curve fits are different. i Mean P2/P1 at 40 ms IPI vs mean peak 1p [DA]o evoked by optical stimulation in CPu (filled) and NAc (open circles). Two-way ANOVA with Bonferroni’s test for post hoc comparisons except where stated otherwise: *P < 0.05, **P < 0.01, ***P < 0.001. Error bars are ± SEM. Source data are provided as a Source Data file
